Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

聚乳酸（PLA）沙障凹曲面及沉积物粒度特征研究   总被引：1，自引：0，他引：1       下载免费PDF全文

王艺钊  原伟杰  丁国栋  虞毅  高广磊  章尧想  王陇 《干旱区地理》2020,43(3):671-678

在乌兰布和沙漠平坦沙地布设1 m×1 m 、1.5 m×1.5 m和2 m×2 m规格的聚乳酸(PLA)沙袋格状沙障，在风季后利用插钎法和激光衍射法分别测量障格内部凹曲面形态、测定表面0~3 cm沉积物粒度组成，分析不同规格沙障内部凹曲面形态特征、蚀积效应，计算平均粒径、标准偏差等粒度参数。结果表明：3种规格PLA沙障内部均能发育成稳定凹曲面，凹曲面深度随着规格的增大而逐渐增加，蚀积系数则逐渐减小，保持在1/12~1/10之间；障格内不同方位蚀积剖面曲线基本符合二次函数，但蚀积强度差异较大；1 m×1 m和1.5 m×1.5 m规格沙障处于堆积状态，堆积强度分别是侵蚀强度的50.1倍和5.6倍，易发生沙埋；2 m×2 m 规格障内单位体积蚀积量为0.02 g·cm^-3，接近蚀积平衡状态，有利于长期发挥沙障防护效益；各规格障内沉积物均以极细沙和细沙为主，除1 m×1 m规格外，PLA沙障的阻滞作用使得极细颗粒含量百分比迅速下降。综合考虑沙障铺设成本和防护效果，得出在平坦沙地或者弱风区域内适合布设2 m×2 m 规格PLA沙障，研究结果可为PLA沙障在防沙工程中的合理应用提供科学参考。  相似文献   

西部省会城市新型城镇化水平综合测度研究          下载免费PDF全文

邹亚锋  李亚静  袁志鸿 《干旱区地理》2020,43(6):1612-1621

新型城镇化是中国经济社会健康、稳定、可持续发展的根本途径，也是消除中国城乡社会经 济“二元结构”的根本出路。根据新型城镇化的内涵，从人口、经济发展、生活质量、基础设施、资源 环境、城乡统筹 6 个方面，构建了包含 24 项指标的新型城镇化水平综合测度指标体系，运用熵值 法，对西部 11 个省会城市 2005—2015 年的城镇化发展水平进行综合测度。研究表明：西部省会城 市的新型城镇化发展水平存在显著差异，总体上可分为“高等水平”、“中等水平”、“低等水平”3 个 级别，基础设施建设是造成差异的主要驱动力。各城市不同年间的城镇化单项水平影响因素呈现 多元化，在今后的发展中应有所侧重，同时兼顾城镇化质量，推动城乡协调发展。  相似文献   

丝绸之路遗产诠释与重游意愿影响关系研究     

王镜  蔡佳佳  原燕妮 《世界地理研究》2020,29(6):1260-1271

为探究世界遗产丝绸之路沿线遗址点遗产诠释与重游意愿的影响关系，研究将遗产诠释分为现场遗产解说与非现场遗产传播两部分，并以旅游体验为中介变量，游客特征为调节变量，构建“遗产诠释-旅游体验-重游意愿”的结构关系模型。研究表明：（1）非现场遗产传播正向影响现场遗产解说；（2）遗产诠释需要通过深刻的旅游体验才能更好地影响重游意愿；（3）遗产诠释与重游意愿的影响关系部分受游客性别和文化程度的调节，其中非现场遗产传播更能在高文化程度的游客中发挥其对重游意愿的影响作用，同时更能在男性游客中发挥其对旅游体验的影响作用。  相似文献   

珠三角基塘系统研究回顾及展望   总被引：2，自引：0，他引：2  

龚建周  蒋超  胡月明  杨颢 《地理科学进展》2020,39(7):1236-1246

基塘系统是中国劳动人民独创的低洼地利用方式和生态循环农业模式,珠三角基塘系统在历史上曾起到重要的作用,但目前其面积萎缩、生态功能退化。为了推动珠三角传统农业文化遗产的保护、促进区域人地关系协调发展,论文结合文献计量学方法对珠三角基塘系统研究文献进行梳理和概括,发现在基(田)塘生产力、区域水陆生态农业循环模式规律、社会经济发展与传统农业文化遗产保护的关系等方面已经展开研究;但是珠三角基塘系统演变机理研究比较薄弱,区域特色的农业文化遗产保护途径探索还鲜见。建议未来加强珠三角基塘系统演变机理、系统重构技术、农业文化遗产保护价值与途径的研究,以此深化基塘系统理论及实践研究,为协同区域农业生产格局与农业生态系统耦合关系提供支撑。  相似文献   

基于树木地貌法重建崩塌历史事件——-以川东华蓥山石林为例     

王锐  马超  王云琦  王玉杰 《山地学报》2020,38(1):132-141

了解崩塌活动史与区域气候变化之间的关系对于掌握区域山地灾害形成规律,预测未来发展趋势具有非常重要的作用。利用树木地貌法可以准确掌握山地灾害发生频率,并为定量分析气候变化对山地灾害发生发展趋势提供支撑。本文以川东华蓥山石林景区一大型崩塌为研究对象,通过杉木树木年轮、降水和气温数据对该崩塌活动时间进行分析。结果表明:该崩塌可能共有四个活动年份,2015年为大规模活动年份,2008年、2012年和2016年为小规模活动年份,崩塌活动的季节主要集中在早材期间(4月—9月)。基于滚石冲击树干产生的愈伤组织和标准木年轮对比表明:树木在受到滚石冲击后,都表现为生长抑制,且树木年轮宽度要滞后滚石活动1年。参考树木与干扰树木年轮宽度和月降雨量没有相关性,但干扰树木年轮与月平均气温(4月—9月)呈负相关关系。本研究结果可以为研究区内山地灾害活动史与气候变化关系提供支撑,尤其是为研究区域降雨诱发的崩塌活动规律提供借鉴。  相似文献   

湿地土壤和沉积物异化铁还原过程研究进展     

朱晓艳  袁宇翔  宋长春  姜明 《湿地科学》2020,18(1):122-128

异化铁还原是湿地土壤和沉积物中重要的生物地球化学过程,也是有机质矿化的主要途径之一。湿地干湿交替等过程会使土壤的氧化还原状态发生改变,影响铁元素及与其相关的元素的迁移和转化。总结了湿地土壤和沉积物中异化铁还原过程及其与碳、磷、硫等元素在生物地球化学循环关键过程中的相互作用,阐述了湿地土壤和沉积物中异化铁还原过程对微量金属元素迁移和转化的影响,分析了影响湿地土壤和沉积物异化铁还原过程的主要环境因子。未来相关研究应集中于湿地土壤和沉积物中异化铁还原微生物分析和纯化、不同有机质形式对异化铁还原过程的影响以及异化铁还原对土壤有机质矿化的贡献。  相似文献   

Source-independent wave-equation based microseismic source location using traveltime inversion          下载免费PDF全文

Songqin Nong  Chao Huang  Liangguo Dong 《地震科学（英文版）》2018,31(2):100-109

A new source location method using wave-equation based traveltime inversion is proposed to locate microseismic events accurately. With a sourceindependent strategy, microseismic events can be located independently regardless of the accuracy of the source signature and the origin time. The traveltime-residuals-based misfit function has robust performance when the velocity model is inaccurate. The new Fréchet derivatives of the misfit function with respect to source location are derived directly based on the acoustic wave equation, accounting for the influence of geometrical perturbation and spatial velocity variation. Unlike the mostly used traveltime inversion methods, no traveltime picking or ray tracing is needed.Additionally, the improved scattering-integral method is applied to reduce the computational cost. Numerical tests show the validity of the proposed method.  相似文献   

Carbon pools and fluxes in the China Seas and adjacent oceans     

Nianzhi Jiao  Yantao Liang  Yongyu Zhang  Jihua Liu  Yao Zhang  Rui Zhang  Meixun Zhao  Minhan Dai  Weidong Zhai  Kunshan Gao  Jinming Song  Dongliang Yuan  Chao Li  Guanghui Lin  Xiaoping Huang  Hongqiang Yan  Limin Hu  Zenghu Zhang  Long Wang  Chunjie Cao  Yawei Luo  Tingwei Luo  Nannan Wang  Hongyue Dang  Dongxiao Wang  Si Zhang 《中国科学:地球科学(英文版)》2018,61(11):1535-1563

The China Seas include the South China Sea, East China Sea, Yellow Sea, and Bohai Sea. Located off the Northwestern Pacific margin, covering 4700000 km~2 from tropical to northern temperate zones, and including a variety of continental margins/basins and depths, the China Seas provide typical cases for carbon budget studies. The South China Sea being a deep basin and part of the Western Pacific Warm Pool is characterized by oceanic features; the East China Sea with a wide continental shelf, enormous terrestrial discharges and open margins to the West Pacific, is featured by strong cross-shelf materials transport; the Yellow Sea is featured by the confluence of cold and warm waters; and the Bohai Sea is a shallow semiclosed gulf with strong impacts of human activities. Three large rivers, the Yangtze River, Yellow River, and Pearl River, flow into the East China Sea, the Bohai Sea, and the South China Sea, respectively. The Kuroshio Current at the outer margin of the Chinese continental shelf is one of the two major western boundary currents of the world oceans and its strength and position directly affect the regional climate of China. These characteristics make the China Seas a typical case of marginal seas to study carbon storage and fluxes. This paper systematically analyzes the literature data on the carbon pools and fluxes of the Bohai Sea,Yellow Sea, East China Sea, and South China Sea, including different interfaces(land-sea, sea-air, sediment-water, and marginal sea-open ocean) and different ecosystems(mangroves, wetland, seagrass beds, macroalgae mariculture, coral reefs, euphotic zones, and water column). Among the four seas, the Bohai Sea and South China Sea are acting as CO_2 sources, releasing about0.22 and 13.86–33.60 Tg C yr~(-1) into the atmosphere, respectively, whereas the Yellow Sea and East China Sea are acting as carbon sinks, absorbing about 1.15 and 6.92–23.30 Tg C yr~(-1) of atmospheric CO_2, respectively. Overall, if only the CO_2 exchange at the sea-air interface is considered, the Chinese marginal seas appear to be a source of atmospheric CO_2, with a net release of 6.01–9.33 Tg C yr~(-1), mainly from the inputs of rivers and adjacent oceans. The riverine dissolved inorganic carbon (DIC) input into the Bohai Sea and Yellow Sea, East China Sea, and South China Sea are 5.04, 14.60, and 40.14 Tg C yr~(-1),respectively. The DIC input from adjacent oceans is as high as 144.81 Tg C yr~(-1), significantly exceeding the carbon released from the seas to the atmosphere. In terms of output, the depositional fluxes of organic carbon in the Bohai Sea, Yellow Sea, East China Sea, and South China Sea are 2.00, 3.60, 7.40, and 5.92 Tg C yr~(-1), respectively. The fluxes of organic carbon from the East China Sea and South China Sea to the adjacent oceans are 15.25–36.70 and 43.93 Tg C yr~(-1), respectively. The annual carbon storage of mangroves, wetlands, and seagrass in Chinese coastal waters is 0.36–1.75 Tg C yr~(-1), with a dissolved organic carbon(DOC) output from seagrass beds of up to 0.59 Tg C yr~(-1). Removable organic carbon flux by Chinese macroalgae mariculture account for 0.68 Tg C yr~(-1) and the associated POC depositional and DOC releasing fluxes are 0.14 and 0.82 Tg C yr~(-1), respectively. Thus, in total, the annual output of organic carbon, which is mainly DOC, in the China Seas is 81.72–104.56 Tg C yr~(-1). The DOC efflux from the East China Sea to the adjacent oceans is 15.00–35.00 Tg C yr~(-1). The DOC efflux from the South China Sea is 31.39 Tg C yr~(-1). Although the marginal China Seas seem to be a source of atmospheric CO_2 based on the CO_2 flux at the sea-air interface, the combined effects of the riverine input in the area, oceanic input, depositional export,and microbial carbon pump(DOC conversion and output) indicate that the China Seas represent an important carbon storage area.  相似文献   

秦皇岛近岸海域绿藻微观繁殖体的分布以及在绿潮形成中的作用   总被引：2，自引：0，他引：2  

韩红宾  宋伟  王宗灵  丁德文  袁超  张学雷  李艳 《海洋学报(英文版)》2019,38(8):72-77

2015年以来，秦皇岛近岸海域暴发了绿潮，对北戴河旅游区的环境和生态系统造成了严重影响。绿藻微观繁殖体在绿潮的形成过程中起到重要作用，主要包括孢子、配子、幼苗和营养片段。绿藻微观繁殖体作为绿潮的“种源”，其分布规律可以反映绿潮的“藻源”位置。本研究于2016年4-9月和2017年1月对秦皇岛近岸海域绿藻微观繁殖体的调查，探究了其分布规律以及生物量变化。结果显示，绿藻微观繁殖体主要分布在近岸海域，由近岸向远岸海域逐渐降低。绿藻微观繁殖体的数量在7、8月份最高，在冬季最低。受绿潮影响严重的海域微观繁殖体数量高于其它海域。秦皇岛近岸海域的绿藻微观繁殖体为该海域绿潮的种源，其分布规律表明秦皇岛近岸海域绿潮起源于本地。  相似文献